Table 7.2 Molecular Probes goat anti-mouse isotype-specific antibodies.

Fluorophore
Abs/Em *
Goat Anti-Mouse Isotype-Specific Antibodies †
IgG11) IgG2a2a) IgG2b2b) IgG33)
Alexa Fluor 350 346/442 A21120 A21130 A21140  
Fluorescein 494/518 A10530      
Alexa Fluor 488 495/519 A21121 A21131 A21141 A21151
Alexa Fluor 546 556/573 A21123 A21133 A21143  
Alexa Fluor 555 555/565 A21127 A21137 A21147  
R-phycoerythrin 496, 546, 565/578 P21129 P21139 P21149  
Alexa Fluor 568 578/603 A21124 A21134 A21144  
Alexa Fluor 594 590/617 A21125 A21135 A21145 A21155
Alexa Fluor 633 632/647 A21126 A21136 A21146  
Allophycocyanin 650/660 A10541 A10686    
Alexa Fluor 647 650/668 A21240 A21241 A21242  
Alexa Fluor 680 679/702 A31562 A31563 A31564  
Horseradish peroxidase NA A10551 A10685    
Biotin NA A10519      
Unlabeled NA A10538      
* Approximate absorption (Abs) and fluorescence emission (Em) maxima, in nm. † The Alexa Fluor goat anti-mouse isotype-specific antibodies have been cross-adsorbed against mouse IgM, IgA, pooled sera, purified human paraproteins and the other isotypes to minimize crossreactivity. NA = Not applicable.