Table 20.4—Reactive pH indicator dyes.

pH Indicator Preferred Reactive Form
BCECF BCECF (B1151, Probes Useful at Near-Neutral pH—Section 20.2) *
Carboxyfluorescein 5-(and 6-)carboxyfluorescein, succinimidyl ester (C1311, Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5)
6-JOE 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester (C6171MP, Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5)
Naphthofluorescein 5-(and 6-)carboxynaphthofluorescein, succinimidyl ester (C653)
Oregon Green 488 Oregon Green 488 carboxylic acid, succinimidyl ester (O6147, O6149)
Oregon Green 514 Oregon Green 514 carboxylic acid, succinimidyl ester (O6139)
pHrodo pHrodo succinimidyl ester (P36600)
SNARF-1 SNARF-1 carboxylic acid, acetate, succinimidyl ester (S22801)
5-(and 6-)carboxy SNARF-1 (C1270, Probes Useful at Near-Neutral pH—Section 20.2) *
SNARF-4F SNARF-4F carboxylic acid (S23920, Probes Useful at Near-Neutral pH—Section 20.2) *
SNARF-5F SNARF-5F carboxylic acid (S23922, Probes Useful at Near-Neutral pH—Section 20.2) *
* Carboxylic acids require activation with EDAC/NHSS before reaction with amines (Introduction to Amine Modification—Section 1.1).