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Table 12.2 Spectral characteristics of the MitoTracker probes.

Cat. No. MitoTracker Probe Abs * (nm) Em * (nm) Oxidation State
M7514 MitoTracker Green FM † 490 516 NA
M7510 MitoTracker Orange CMTMRos 551 576 Oxidized
M7511 MitoTracker Orange CM-H2TMRos 551 ‡ 576 ‡ Reduced
M7512 MitoTracker Red CMXRos 578 599 Oxidized
M7513 MitoTracker Red CM-H2XRos 578 ‡ 599 ‡ Reduced
M22425 MitoTracker Red FM 581 644 NA
M22426 MitoTracker Deep Red FM 644 665 NA
* Absorption (Abs) and fluorescence emission (Em) maxima, determined in methanol; values may vary somewhat in cellular environments. † MitoTracker Green FM is nonfluorescent in aqueous environments. ‡ These reduced MitoTracker probes are not fluorescent until oxidized. NA = Not applicable.
For Research Use Only. Not for use in diagnostic procedures.