Table 23.4 Spectroscopic data for components of the Reference Dye Sampler Kit.

Component Solvent Abs (nm) * Em (nm) * QY †
Quinine sulfate Water 347 ‡ 455 ‡ 0.55 ‡
Fluorescein Water 490 § 514 § 0.92 §
5-CTMR ** Methanol 542 568 0.68
Sulforhodamine 101 Ethanol 578 605 0.90
Nile blue Ethanol 636 665 0.27
* Approximate absorbance (Abs) and fluorescence emission (Em) maxima. †  Fluorescence quantum yield at 22°C. ‡  Standard values in 0.5 M H2SO4. §  Standard values in 0.1 M NaOH. ** CTMR = Carboxytetramethylrhodamine.