Table 17.2 Spectroscopic properties of MANT-ATP in aqueous solution (pH 8).

Parameter Value Notes
Absorption maximum356 nmStronger absorption at shorter wavelengths (λmax = 255 nm)
Molar extinction coefficient (εmax)5800 cm-1M-123,000 cm-1M-1 at 255 nm
Fluorescence emission maximum448 nmShifts 10–20 nm shorter in nonpolar solvents and upon binding to most proteins
Fluorescence quantum yield0.22Increases in nonpolar solvents and upon binding to most proteins