Table 17.2 Spectroscopic properties of MANT-ATP in aqueous solution (pH 8).

Parameter Value Notes
Absorption maximum 356 nm Stronger absorption at shorter wavelengths (λmax = 255 nm)
Molar extinction coefficient (εmax) 5800 cm-1M-1 23,000 cm-1M-1 at 255 nm
Fluorescence emission maximum 448 nm Shifts 10–20 nm shorter in nonpolar solvents and upon binding to most proteins
Fluorescence quantum yield 0.22 Increases in nonpolar solvents and upon binding to most proteins