Table 9.2 SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains.

Dye (Cat #) Ex/Em * Major Applications Features
SYPRO Ruby protein gel stain (S12000, S12001, S21900) 280, 450/610 2D gels, IEF gels, 1D SDS-PAGE
  • High sensitivity (1–2 ng/band; comparable to silver staining)
  • Linear quantitation range over three orders of magnitude
SYPRO Orange protein gel stain (S6650, S6651) 300, 470/570 1D SDS-PAGE
  • Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining)
  • Little protein-to-protein variability
  • Linear quantitation range over three orders of magnitude
SYPRO Red protein gel stain (S6653, S6654) 300, 550/630 1D SDS-PAGE
  • Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining)
  • Little protein-to-protein variability
  • Linear quantitation range over three orders of magnitude
SYPRO Tangerine protein gel stain (S12010) 300, 490/640 1D SDS-PAGE, blotting applications, zymography, electroelution
  • Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining)
  • Little protein-to-protein variability
  • Linear quantitation range over three orders of magnitude
  • No fixation required
Coomassie Fluor Orange protein gel stain (C33250, C33251) 300, 470/570 1D SDS-PAGE
  • Premixed and ready-to-use solution
  • Very good sensitivity (8 ng/band; higher than Coomassie brilliant blue staining)
  • Little protein-to-protein variability
  • Linear quantitation range over at least two orders of magnitude
All SYPRO gel and blot stains are compatible with Edman sequencing and mass spectrometry. * Excitation (Ex) and emission (Em) maxima, in nm. For maximum sensitivity, use excitation sources and optical filters matched to these values.