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Measure Cytokines That Drive Th2 Differentiation

  • Easy-to-run assays—4 hours of incubation time
  • Convenient precoated, removable 8-well strips
  • Quantitative, sensitive results

Interleukins are cytokines involved in the immune network with pro- and anti-inflammatory properties. IL-4 is produced by the Th2 subtype of CD4+ T lymphocytes and by mast cell precursors. IL-4 and IL-2 have been shown to be important drivers in Th2 differentiation.

The ELISA and multiplex bead-based assays using the Luminex® platform offer reliable, easy methods to quantitatively measure cytokines. The assay can be run and quantitative results can be obtained within a day in a 96-well plate format. Convenient strip-well plates also provide flexibility to run as few samples as needed, allowing you to assay precious samples.


PBMCs stimulated with: IL-4 measured
 PHA (1 µg/mL)  14–26 pg/mL
 LPS (25 µg/mL) + PHA (5 µg/mL)  18–40 pg/mL
 Ionomycin (0.1 µg/mL) + PMA (3 ng/mL)  32–70 pg/mL

 Table 1. IL-4 measured in peripheral blood mononuclear cells (PBMCs) following stimulation.


  Figure 1. Representative standard curves show the dynamic range and sensitivity of the IL-4 Human ELISA Kit  (normal) and the IL-4 Human Ultrasensitive ELISA Kit.

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.

New IL-4 and STAT6 ABfinity™ Recombinant Antibodies for Studying Th2 Differentiation

  • ABfinity™ recombinant antibodies are screened to be sensitive and specific
  • Enable consistent performance for your assays
  • Consistent product from lot to lot

ABfinity™ recombinant rabbit antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. Lot-to-lot variability, commonly a problem with polyclonal products, is minimized with ABfinity™ antibodies. In addition to the monoclonal versions of our ABfinity™ antibodies, we offer oligoclonal versions of several of these specificities. Oligoclonal ABfinity™ products are a mixture of recombinant monoclonal antibodies and offer the improved signal strength you get with polyclonal antibodies, combined with the highly reproducible results you get from monoclonals.

Th2 cell differentiation is initiated through IL-4 stimulation of the IL-4 receptor in the presence of T cell activating receptor (TCR) crosslinking in naive T cells. IL-4R signaling, mediated by STAT6, results in transcription of IL-4 and GATA3. GATA3 remodels chromatin, driving T cells towards the Th2 lineage.



ABfinity™ Stem Cell Antibody Selection Guide

For Research Use Only. Not for use in diagnostic procedures.

Gibco® Recombinant IL-4

  • High biological activity
  • High purity
  • Proven compatibility with Gibco® media

Interleukin 4 (IL-4) is a key cytokine in inducing Th2 differentiation. IL-4 interacts with the IL-4 receptor, activating the STAT6 pathway, which in turn leads to IL-4 transcription, setting up an autocrine loop. We offer Gibco® Recombinant IL-4 from human, mouse, and swine for use in studying Th2 differentiation.

Gibco® cytokines and growth factors are high-purity recombinant proteins with high bioactivity. Gibco® growth factors are of the highest quality because each protein is analyzed for purity along with structural homogeneity to help ensure a biologically active protein is produced. And Gibco® cytokines and growth factors have been bioassayed with Gibco® media. Get your cytokines and growth factors from the media company you use and trust.


Gibco® Recombinant IL-4 Selection Guide

Role of Oxidative Stress In Th2 Differentiation

  • Optimized probes for detection of oxidative stress in live cells
  • Simple 30-minute protocol that can be multiplexed with other compatible live-cell dyes
  • Compatible with various platforms such as live-cell fluorescence imaging, high-content screening (HCS), flow cytometry, high-throughput screening (HTS)

CellROX® oxidative stress reagents are fluorogenic probes designed to reliably measure reactive oxygen species (ROS) in live cells. The cell-permeant reagents are nonfluorescent (or very weakly fluorescent) while in a reduced state. Upon oxidation, they produce a strong fluorescent signal. With these reagents, researchers can detect oxidative stress in the red, orange, and green channels of their instruments.

Oxidative stress results from an imbalance between the production of reactive oxygen species (ROS) and the ability of cells to scavenge them. In addition to the role oxidative stress plays in the progression of many diseases, ROS also appears to drive T cells toward the Th2 phenotype (J Immunol 176:2765 (2006)). CellROX® reagents offer a simple live-cell workflow for measuring oxidative stress that can be adapted to various platforms and benchtop instruments, such as the Tali® Image-Based Cytometer, FLoid™ Cell Imaging Station, and Attune® Acoustic Focusing Cytometer.


  Figure 2. Imaging oxidative stress with CellROX® Green ReagentHuman osteosarcoma (U2OS) cells were treated with 100 µM menadione for 1 hr.  A staining solution containing 5 µM CellROX® Green, 300 nM MitoTracker® Deep Red, and 2 drops/mL of NucBlue™ Live Cell Stain was applied for 30 min at 37°C.  After 25 min of incubation in the staining solution, 5 µg/mL of CellMask™ Orange Plasma Membrane Stain was added directly to cells for the last 5 min of incubation. Cells were washed and imaged with Live Cell Imaging Solution.


CellRox® Reagent Selection Guide

Reagent Absorption/ Emission(Color) Live-Cell Compatible? Formaldehyde- Fixable? Detergent-Resistant? Platforms Cat. No.
CellROX® Deep Red Reagent 644/665 (deep red) Yes Yes No Imaging, HCS, HTS, flow cytometry, Attune® C10422
CellROX® Orange Reagent 545/565 (orange) Yes No No Imaging, HCS, flow cytometry, Tali® C10443
CellROX® Green Reagent 485/520 (green) Yes Yes Yes Imaging, HCS, HTS, flow cytometry, Tali®, FLoid™, Attune® C10444
CellROX® Variety Pack Contains one vial (50 µL) each of CellROX® Green Reagent, CellROX® Orange Reagent, and CellROX® Deep Red Reagent C10448

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