Apply CRISPR-Cas9 gene editing to high-throughput screening
The CRISPR-Cas9 gene editing system provides an efficient approach for specific, complete, and permanent knockout of gene expression, making it a potent research tool for determining key players in specific biological pathways. The new Invitrogen™ LentiArray™ CRISPR libraries extend CRISPR-Cas9 technology into high-throughput applications for functional genomic screening. LentiArray libraries enable the interrogation of hundreds or thousands of genes in a single experiment with:
- Advanced guide RNA (gRNA) designs for maximum knockout efficiency without sacrificing specificity
- Up to 4 high-quality gRNAs per gene target, for efficient knockout in a wide variety of cell types
- Two choices for delivery—high-titer, ready-to- use lentivirus, or glycerol stocks of E. coli containing lentiviral plasmids
- A complete set of controls and lentiviruses against single-gene targets to support pre-screen assay development and rapid post-screen hit validation
- 19 defined libraries and custom options available, enabling screens of defined gene sets or unbiased surveys of the whole genome (Table 1)
LentiArray library specifics
For example, the LentiArray Human Whole Genome CRISPR Library targets 18,453 genes with up to 4 gRNAs per gene target (pooled in a single well), for a total of 73,812 gRNAs. The gene targets within this library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database or the HUGO Gene Nomenclature Committee (HGNC). LentiArray CRISPR libraries are constructed using our proprietary CRISPR gRNA design algorithm, which incorporates the latest gRNA design research; gRNAs are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene. Libraries are delivered as 200 μL of ready-to-use lentiviral particles per gene target at a titer of 1 x 106 TU/mL (functional titer determined by antibiotic resistance) and are also available as glycerol stocks (Table 1).
Table 1. Available defined libraries, supporting focused high-throughput screens as well as unbiased whole genome surveys.
|Product||No. of genes||Cat. No.
(ready to use)
|LentiArray™ Human Whole Genome CRISPR Library||18,453||A31949||A32185|
|LentiArray™ Human Druggable Genome CRISPR Library||10,128||A31948||A32184|
|LentiArray™ Human Apoptosis CRISPR Library||904||A31940||A32176|
|LentiArray™ Human Cancer Biology CRISPR Library||510||A31933||A32169|
|LentiArray™ Human Cell Cycle CRISPR Library||1,444||A31936||A32172|
|LentiArray™ Human Cell Surface CRISPR Library||778||A31943||A32179|
|LentiArray™ Human DNA Damage Response CRISPR Library||561||A31946||A32182|
|LentiArray™ Human Drug Transport CRISPR Library||98||A31941||A32177|
|LentiArray™ Human Epigenetics CRISPR Library||396||A31934||A32170|
|LentiArray™ Human GPCR CRISPR Library||446||A31947||A32183|
|LentiArray™ Human Ion Channel CRISPR Library||328||A31942||A32178|
|LentiArray™ Human Kinase CRISPR Library||840||A31931||A32167|
|LentiArray™ Human Membrane Trafficking CRISPR Library||141||A31937||A32173|
|LentiArray™ Human Nuclear Hormone Receptor CRISPR Library||47||A31939||A32175|
|LentiArray™ Human Phosphatase CRISPR Library||288||A31932||A32168|
|LentiArray™ Human Protease CRISPR Library||475||A31944||A32180|
|LentiArray™ Human Transcription Factor CRISPR Library||1,817||A31938||A32174|
|LentiArray™ Human Tumor Suppressor CRISPR Library||716||A31945||A32181|
|LentiArray™ Human Ubiquitin CRISPR Library||943||A31935||A32171|
Learn more about the LentiArray libraries
LentiArray CRISPR libraries are delivered in an arrayed format compatible with existing high-throughput screening infrastructure and have been designed and constructed to provide a flexible system that doesn’t impose limitations on your assay design. They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA from separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells. Explore the LentiArray CRISPR libraries and find out how CRISPR-Cas9 technology can expand your screening capabilities.
For Research Use Only. Not for use in diagnostic procedures.