Low protein-to-protein variation in the Qubit® protein assay
Low protein-to-protein variation in the Qubit® protein assay (Q33211, Q33212). Solutions of the following proteins were prepared, diluted, and assayed in the Qubit® protein assay: bovine serum albumin (BSA), chicken-egg ovalbumin, chicken-egg lysozyme, bovine-milk ß-casein, equine myoglobin, bovine-milk a-casein, porcine pepsin, mouse immunoglobulin (IgG), and calf-thymus histone. Fluorescence was measured at 485/590 nm and plotted versus the mass of the protein sample. At 3 µg, the fluorescence variation was 12.4%, or 8.7% excluding the basic histone protein. Background fluorescence has not been subtracted.