Cell proliferation detected with Click-iT® EdU and counter stained with anti-LC3B and a goat anti-rabbit Alexa Fluor® 647 secondary antibody.
HeLa cells were treated with 30 µM chloroquine and cultured overnight. The following day, the cells were fed 10 µM EdU under regular growth conditions for one hour and then fixed and permeabilized. EdU was used to was visualize proliferating cells using The Click-iT® EdU Alexa Fluor® 488 Imaging kit (pink). Cells were counter stained with 0.5 µg/mL anti-LC3B with a goat anti rabbit Alexa Fluor® 647 secondary (Green), mouse anti alpha tubulin with a goat anti mouse Alexa Fluor® 555 secondary (Cyan) and 1 µg/mL Hoechst 33342 (Blue). Cells were imaged on a Molecular Devices ImageXpress High content imager.
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›
Endothelial cells. Go ›
1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
Performance of the Qubit® dsDNA HS Assay Go ›
Low protein-to-protein variation in the Qubit® protein assay Go ›
Muntjac fibroblast labeled with probes for actin and the nucleus. Go ›
Multicolor fluorescence analysis of muntjac fibroblasts. Go ›
Proliferating cells in rat ileum using Click-iT® EdU. Go ›