Normalization with housekeeping genes on a single blot
Simultaneous detection of a protein of interest and a loading control on a single blot using WesternDot® secondary antibodies. A western blot containing a dilution series of recombinant AKT in a background of Jurkat lysate at a constant loading of 5 µg total protein. The blot was probed with mouse anti-AKT and rabbit anti-GAPDH, followed by WesternDot™ 655 goat anti-mouse (Cat. no. W10813, pseudocolored green) and WesternDot™ 800 goat anti-rabbit (Cat. No. W10816, pseudocolored red) conjugates and imaged using the Fujifilm® FLA-9000 imager.