HL-60 cells in a comet assay.
Comet assay with SYBR® Green I Nucleic Acid Gel Stain (Cat. No. S7563, S7567, S7585). DNA fragmentation associated with oxidative DNA damage was visualized using Trevigen's CometAssay kit. HL-60 cells were treated with hydrogen peroxide and immobilized onto a Trevigen CometSlide for analysis. The cells were gently lysed, washed, and treated with endonuclease. Slides were subjected to electrophoresis in alkaline electrophoresis buffer and stained with SYBR® Green I stain.