Live CRE BAG 2 cells.
Live CRE BAG 2 cells loaded with the beta-galactosidase substrate from the DetectaGene Green CMFDG lacZ Gene Expression Kit (Cat. No. D2920). The substrate was loaded into cells with the aid of the Influx™ pinocytic cell-loading reagent (Cat. No. I14402), a component of the DetectaGene™ Green Kit. The cells were then stained with LysoTracker® Red DND-99 (Cat. No. L7528) to differentiate lysosomal from cytoplasmic localization of the green-fluorescent CMFDG product, as well as to illustrate the selective rupture of only the Influx™ reagent–induced pinocytic vesicles. The double-exposure image was acquired using bandpass filters appropriate for fluorescein and Texas Red® dye.
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain. Go ›
Madin-Darby canine kidney cells. LysoSensor Yellow/Blue DND-160. Go ›
Zymographic detection of ß-D-glucuronidase activity. Go ›
Bovine pulmonary artery endothelial cells (BPAEC). Go ›
lacZ-negative (NIH 3T3) and lacZ-positive (CRE BAG 2) cells. Go ›
Adherent CRE BAG 2 cells. Alexa Fluor 594 hydrazide and Influx pinocytic cell-loading reagent. Go ›
Figure 5: IgG binding capacity vs. salt concentration Go ›
Figure 4: Resolution as a function of protein loading Go ›