Simultaneous detection of three gene targets in a whole-mount Drosophila embryo by fluorescence in situ hybridization.
Pseudocolored green-fluorescent labeling represents a fluorescein-labeled cRNA probe detected using a rabbit anti-fluorescein/Oregon Green® primary antibody (Cat. No. A889) and an Alexa Fluor® 488 dye–labeled anti-rabbit secondary antibody (Cat. No. A11008). Pseudocolored yellow- and red-fluorescent labeling represents a biotinylated cRNA probe detected using HRP–streptavidin and Alexa Fluor® 568 tyramide (TSA Kit #24, Cat. No. T20934). Pseudocolored blue-fluorescent labeling represents a digoxigenin-labeled cRNA probe detected using a mouse anti-digoxigenin primary antibody in conjunction with an Alexa Fluor® 647 dye–labeled anti-mouse secondary antibody (Cat. No. A21235). The image was contributed by Ethan Bier and David Kosman, University of California, San Diego.
Simultaneous detection of expression of five genes in a whole mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes Go ›
Labeled paint probes hybridized to human metaphase chromosomes Go ›
Immunocytochemistry using Alpha-Tubulin Monoclonal Antibody, Mouse Go ›
Tyramide signal amplification of immunofluorescent staining in mouse brain sections. Go ›