Higher protein yields achieved with the MagicMedia™ E. coli Expression Medium
Eight different human ORFs were cloned into the Champion™ pET300/NT-DEST vector using Gateway® cloning. Positive clones were transformed into BL21(DE3) E. coli. The clones were expressed in LB + IPTG (lane 1), ready-to-use liquid MagicMedia™ medium (lane 2), or MagicMedia™ medium prepared from powder (lane 3). Two hundred microliters of each culture were lysed and analyzed on a Coomassie-stained NuPAGE® Novex® 4-12% Bis-Tris Protein Gel. M: SeeBlue® Protein Standard.