Forward (FSC) and side (SSC) scatter patterns after acquisition with low and high FSC threshold value
Figure 1: Forward (FSC) and side (SSC) scatter patterns after acquisition with a low FSC threshold value. Platelets are clearly visible in the low FSC-SSC region. Figure 2: FSC and SSC patterns after acquisition with a higher FSC threshold value. Platelets have been eliminated from view.
High-Yield Lyse is a premixed, fixative-free erythrocyte lysing solution that can be used to eliminate red cells from whole blood for flow cytometric analysis with minimal loss of rare blood cell populations. Using this reagent, lysis of erythrocytes is performed immediately following staining of the blood samples with fluorochrome-conjugated monoclonal antibodies. There is no need for a wash step.