The relaxation of a single, 39 µm–long DNA molecule stained with YOYO®-1 iodide imaged at 4.5 second intervals.
The relaxation of a single, 39 µm–long DNA molecule stained with YOYO®-1 iodide (Cat. no. Y3601) imaged at 4.5 second intervals. After the 1 µm polystyrene sphere was trapped with optical tweezers, the attached DNA was stretched to its full extension in a fluid flow and then allowed to relax upon stoppage of fluid flow due to its entropic elasticity. (Science 264, 822 (1994)) The YOYO®-1 iodide–DNA complex is excited with the 488 nm spectral line of the argon-ion laser and visualized through a 515 nm longpass optical filter using a Hamamatsu SIT camera with image processing. Image contributed by Thomas Perkins, Stanford University.
1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›