Golgi apparatus. BODIPY FL C5-ceramide
Selective staining of the Golgi apparatus using the green-fluorescent BODIPY FL C5-ceramide (D3521) (top panel). At high concentrations, the BODIPY FL fluorophore forms excimers that can be visualized using a red longpass optical filter (bottom panel). The BODIPY FL C5-ceramide accumulation in the trans-Golgi is sufficient for excimer formation J Cell Biol (1991) 113:1267). Images contributed by Richard Pagano, Mayo Foundation.