Reversible binding by DSB-X biotin.
Reversible binding by DSB-X biotin. Microtubules of fixed bovine pulmonary artery endothelial cells were labeled with mouse monoclonal anti–a-tubulin antibody (A11126), detected with either biotin-XX goat anti–mouse IgG antibody (B2763, panel A) or DSB-X biotin goat anti–mouse IgG antibody (D20691, panel B) and visualized with green-fluorescent Alexa Fluor 488 streptavidin (S11223). Nuclei were stained with blue-fluorescent DAPI (D1306, D3571, D21490). After incubating with 10 mM D-biotin (B1595, B20656), the binding between the biotinylated antibody is unaltered (panel C), whereas the streptavidin conjugate has been stripped from the DSB-X biotin–labeled antibody (panel D).
A prometaphase muntjac skin fibroblast stained with Alexa Fluor® 350 phalloidin, an anti–a-tubulin antibody and an anti–cdc6 peptide antibody. Go ›
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›