Animal Health Disease Detection Support—Getting Started
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Optimize your animal health disease detection experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs.
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We recommend running the Xeno Internal Positive Control with our animal health qPCR reagents as a positive control and to help determine whether or not a sample has inhibitors. Many animal health samples will not have a high level or any disease target, so running the Xeno control allows you to be confident that your qPCR reaction worked and that the absence of target signal is due to the true absence of the target instead of a reaction failure. We have Xeno Control Assays labeled with either VIC or LIZ.
We recommend one of the following assays:
- VetMAX Xeno Internal Positive Control—VIC Assay (Cat. No. A29765)
- VetMAX Xeno Internal Positive Control—LIZ Assay (Cat. No. A29766).
The VetMAX Xeno Internal Positive Control is derived from a plasmid source.
The quencher used in these assays is an Eclipse Q quencher. Within the qPCR instrument’s software, choose “none”.
Generally, we recommend using this master mix with standard cycling conditions, but depending on the assay being used, you could use fast cycling conditions with optimization.
For Veterinary Use Only. For In Vitro Use Only