Having difficulties with your experiment?

We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios with your animal health disease detection experiments.

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General

You can try diluting your nucleic acid samples and see if your qPCR signal gets stronger. Alternatively, you can use an extraction control such as the VetMAX Xeno Internal Positive Control we offer (Cat. Nos. A29763, A29764). 

If your Xeno control fails, you could possibly have inhibitors in your samples. You can test this by running the Xeno control directly in a qPCR reaction. You can also run a mock extraction control where you spike the Xeno control into a buffer sample and then perform the nucleic acid extraction procedure you used for your samples. 

There are several reasons that amplification could be delayed. Please see the information in our real-time PCR troubleshooting tool for more details.

If your amplification curves do not have a normal appearance, you may be having an issue with the baseline settings. Please see these examples and suggested solutions in our real-time PCR troubleshooting tool for more details.

There are several reasons for amplification in a NTC well. Please see the information in our real-time PCR troubleshooting tool for more details.

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