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General

If you are using a master mix that has ROXdye provided in a separate tube, use a low ROXdye concentration (50 nM, final well volume). 

No. For multi-plate analysis of gene expression data, please use the Applied Biosystems™ qPCR Analysis Module (RQ application) with your Thermo Fisher Cloud account. 

No. You can use the QuantStudio™ Design and Analysis Desktop Software, or for multi-plate analysis of genotyping data, please use the Applied Biosystems™ qPCR Analysis Module (GT application) with your Thermo Fisher Cloud account. 

Check if the Cloud Monitor is on. Go to Settings --> Maintenance and Service --> Monitoring. Turn CloudSuite Monitor ‘ON’. This needs to be set to ‘ON’, otherwise the instrument will not look out to connect to your account even if you are logged in. 

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