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Flow Cytometry analysis of endogenous Osteopontin (cleaved) was performed on U-2 OS cells labeled with ABfinity? Anti-Osteopontin (cleaved) Recombinant Rabbit Monoclonal Antibody (Product## 702184, 5 ug/ 1M cells) or with rabbit isotype control at 0.5 ug/ml and detected with Goat anti-Rabbit IgG (H+L) Superclonal? Secondary Antibody, (Alexa Fluor® 488 conjugate, Product## A27034, 0.4 ug/ml, 1:2500) as represented by the red and pink histograms respectively. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control. A representative of 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer (4468770).
