Tandem Mass Tag (TMT) reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry (MS). Isobaric TMT reagents share identical strucures, including a peptide-reactive labeling group, a spacer arm and a MS/MS reporter. During the MS/MS analysis, each isobaric tag produces a unique reporter ion that makes quantitation possible. Amine-reactive NHS-ester isotopic duplex or six-plex isobaric TMT tags covalently react with amino termini and lysine residues of peptides and proteins. Cysteine-reactive Tandem Mass Tag (cysTMT) reagents react with reduced thiols of cysteine-containing peptides.
Tandem Mass Tag