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Invitrogen

CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), PE, eBioscience™

19 Published Figures
19 References
View (145) other CD31 (PECAM-1) antibodies
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Data Sheets
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Product Details

Tested Applications
Dilution
Flow Cytometry (Flow)
5 µL (0.125 µg)/test
Published Applications
Flow Cytometry (Flow)
See 19 publications below
Product Specifications
Species Reactivity
Human
Published species
Human, Mouse
Host / Isotype
Mouse / IgG1, kappa
Recommended Isotype Control
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
WM-59 (WM59)
Conjugate
PE
Excitation/Emission Profile
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
RRID
AB_10669160

Product Specific Information

Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.

Applications Reported: The WM-59 (WM59) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This WM-59 (WM59) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Background/Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: adhesion molecule; CD31; CD31 antigen; CD31/EndoCAM; EndoCAM; GPIIA'; PECA1; PECAM-1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1

Gene Aliases: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; PECAM1

UniProt ID: (Human) P16284

Entrez Gene ID: (Human) 5175

Molecular Function: cell adhesion molecule

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