Description: The MAR1-5A3 monoclonal antibody reacts with mouse IFN alpha/beta receptor subunit 1 (IFNAR-1). The type I IFN receptor subunit 1 is co-expressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs, IFN alpha and IFN beta. Type I IFNs are a group of structurally and functionally related proteins that demonstrate antiviral, antiparasitic, and antiproliferative activities.
Applications Reported: This MAR1-5A3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MAR1-5A3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron,2001, Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon- and Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IFN-alpha/beta receptor 1; IFN-alpha/betaR; IFN-R-1; INF-a receptor; Interferon alpha/beta receptor 1; interferon-alpha/beta receptor 1; Type I interferon receptor 1
Gene Aliases: CD118; Ifar; Ifnar; Ifnar1; Ifrc; Infar
UniProt ID: (Mouse) P33896
Entrez Gene ID: (Mouse) 15975