|Flow Cytometry (Flow)||5 µL (0.25 µg)/test|
|Host / Isotype||Mouse / IgG1, kappa|
|Recommended Isotype Control||Mouse IgG1 kappa Isotype Control (P184.108.40.206.1), PE, eBioscience™|
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS, pH 7.2, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: The 2D7 monoclonal antibody recognizes human 2D7 antigen expressed exclusively by human basophils. This human basophil marker is a 7.2-7.5 kDa protein present exclusively in the secretory granules of human basophils. Upon activation, basophils may degranulate, thereby decreasing the amount of 2D7 antigen available for staining. This antibody does not stain mast cells.
Applications Reported: This 2D7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 2D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation and Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This antibody can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
The human 2D7 antigen is expressed exclusively by human basophils. This human basophil marker is a 7.2-7.5 kDa protein present exclusively in the secretory granules of human basophils. Upon activation, basophils may degranulate, thereby decreasing the amount of 2D7 antigen available for staining.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Basophil 2D7 marker; Basophils; Human basophil marker
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