|Tested species reactivity||Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal region of rat YWHAB conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate, Xenopus and zebrafish based on 100% sequence homology.
This antibody is specific for the ~29 kDa 14-3-3 protein. Immunolabeling is blocked by preadsorption of the antibody with the protein used to generate the antibody.
14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A proteomic analysis reveals the interaction of GluK1 ionotropic kainate receptor subunits with Go proteins.
PA1-4647 was used in western blot to determine how kainate receptors activate G-proteins
|Rutkowska-Wlodarczyk I,Aller MI,Valbuena S,Bologna JC,Prézeau L,Lerma J||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:5171)||2015|