Invitrogen

14-3-3 eta Polyclonal Antibody

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14-3-3 eta Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of 14-3-3 eta in Hela cells using a 14-3-3 eta polyclonal antibody (Product # PA5-98163) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

14-3-3 eta Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of 14-3-3 eta in paraffin embedded human skeletal muscle tissue using a 14-3-3 eta polyclonal antibody (Product # PA5-98163) at a dilution of 1:400. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

14-3-3 eta Antibody in Western Blot (WB) — Western Blot analysis of 14-3-3 eta using a 14-3-3 eta Polyclonal antibody (Product # PA5-98163) at a concentration of 4.5 µg/mL. Positive WB detected in: HepG2 whole cell lysate, Mouse kidney tissue, Mouse brain tissue, Mouse thymus tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 29 kDa.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

Assay-Dependent

Immunocytochemistry (ICC)

1:50-1:200

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human 14-3-3 protein eta protein (4-246AA)

Conjugate

Unconjugated

Form

Liquid

Concentration

3.17 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.03% Proclin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 14-3-3 eta; 14-3-3 protein eta; Protein AS1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide

Gene Aliases: YWHA1; YWHAH

UniProt ID: (Human) Q04917, (Mouse) P68510

Entrez Gene ID: (Human) 7533, (Mouse) 22629

Molecular Function: chaperone