Western blot analysis of 14-3-3 isoforms in HEK293 cell extracts using a 14-3-3 gamma polyclonal antibody (Product # PA1-86556) (lane 1), a zeta 14-3-3 (lane 2), a C-epsilon-14-3-3 (lane 3), a N-epsilon-14-3-3 (Lane 4), a eta 14-3-3 (Lane 5). The 14-3-3 isoforms run at 30kDa except epsilon 14-3-3.
|Tested species reactivity||Bovine, Chicken, Human, Mouse, Sheep, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide of acetylated N-terminal amino acids 1-10 of sheep. Peptide sequence: Ac. V D R E Q L V Q K A C of sheep 14-3-3 gamma.|
|Storage buffer||whole serum|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1/800|
|Western Blot (WB)||1:6000-1:12,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Predicted to react with all mammals based on sequence homology.
Heat-mediated antigen retrieval is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is normal brain tissue. In Western blot, this antibody detects a band at ~30kDa in HEK293 cell lysate.
14-3-3 proteins are present as multigene families in most organisms. Signal-induced phosphorylation has the ability to change protein function. Sometimes, however, phosphorylation is not enough to change a protein's function. 14-3-3 proteins play an important role of bringing signal transduction to completion. They regulate many cellular processes that are important in cancer biology, such as apoptosis and cell-cycle checkpoints.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.