Immunofluorescence analysis of 14-3-3 sigma was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled 14-3-3 sigma (1433S01) Mouse Monoclonal Antibody (MA511663) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human 1433 sigma protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11663 targets 14-3-3 sigma in ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human samples.
The MA5-11663 immunogen is recombinant human 1433 sigma protein.
14.3.3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14.3.3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14.3.3 and target proteins are strongly influenced by the phosphorylation state of 14.3.3 and the target protein. 14.3.3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1, and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hypoxia induces an undifferentiated phenotype of oral keratinocytes in vitro.
MA5-11663 was used in western blot to study the effects of hypoxia on the proliferating potential and phenotype of primary human oral keratinocytes
|Kato H,Izumi K,Uenoyama A,Shiomi A,Kuo S,Feinberg SE||Cells, tissues, organs (199:393)||2015|
Growth inhibitory effect of the human NIT2 gene and its allelic imbalance in cancers.
MA5-11663 was used in western blot to study the role of NIT2 gene in cell proliferation and tumorigenesis
|Lin CH,Chung MY,Chen WB,Chien CH||The FEBS journal (274:2946)||2007|
Promoter methylation of PARG1, a novel candidate tumor suppressor gene in mantle-cell lymphomas.
MA5-11663 was used in western blot to characterize a novel tumor suppressor gene and its role in mantle-cell lymphomas
|Ripperger T,von Neuhoff N,Kamphues K,Emura M,Lehmann U,Tauscher M,Schraders M,Groenen P,Skawran B,Rudolph C,Callet-Bauchu E,van Krieken JH,Schlegelberger B,Steinemann D||Haematologica (92:460)||2007|
Identification of 14-3-3sigma as a contributor to drug resistance in human breast cancer cells using functional proteomic analysis.
MA5-11663 was used in western blot to study the role of 14-3-3sigma in drug resistance in human breast cancer cells
|Liu Y,Liu H,Han B,Zhang JT||Cancer research (66:3248)||2006|
Identification of novel target proteins in sebaceous gland carcinoma.
MA5-11663 was used in immunohistochemistry to identify novel therapeutic target proteins in sebaceous gland carcinoma
|Erovic BM,Al Habeeb A,Harris L,Goldstein DP,Kim D,Ghazarian D,Irish JC||Head and neck (35:642)||2013|
14-3-3 sigma is a useful immunohistochemical marker for diagnosing ovarian granulosa cell tumors and steroid cell tumors.
MA5-11663 was used in immunohistochemistry to study the value of immunohistochemical expression of 14-3-3-sigma as a diagnostic marker of ovarian granulosa cell tumors and steroid cell tumors
|Chen L,Yang B||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (32:156)||2013|
Biomarkers of parathyroid carcinoma.
MA5-11663 was used in immunohistochemistry to establish a biomarker panel suitable for the identification of parathyroid carcinomas
|Erovic BM,Harris L,Jamali M,Goldstein DP,Irish JC,Asa SL,Mete O||Endocrine pathology (23:221)||2012|
Proteomic profiling of differential display analysis for human oral squamous cell carcinoma: 14-3-3 ¿ Protein is upregulated in human oral squamous cell carcinoma and dependent on the differentiation level.
MA5-11663 was used in immunohistochemistry to detect the expression of 14-3-3 sigma in oral squamous cell carcinoma
|Hayashi E,Kuramitsu Y,Fujimoto M,Zhang X,Tanaka T,Uchida K,Fukuda T,Furumoto H,Ueyama Y,Nakamura K||Proteomics. Clinical applications (3:1338)||2009|
Immunostaining as an adjunct to cytology for diagnosis of pancreatic adenocarcinoma.
MA5-11663 was used in immunohistochemistry to study whether immunostaining as an adjunct to cytology improves the diagnosis of pancreatic adenocarcinoma
|Agarwal B,Ludwig OJ,Collins BT,Cortese C||Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association (6:1425)||2008|
The clinicopathological and prognostic impact of 14-3-3 sigma expression on vulvar squamous cell carcinomas.
MA5-11663 was used in immunohistochemistry to study the pathological and prognostic significance of 14-3-3 sigma expression fin vulvar squamous cell carcinomas
|Wang Z,Tropè CG,Suo Z,Trøen G,Yang G,Nesland JM,Holm R||BMC cancer (8:null)||2008|
Loss of 14-3-3 sigma protein expression and presence of human papillomavirus type 16 E6 in oral squamous cell carcinoma.
MA5-11663 was used in immunohistochemistry to study the role of 14-3-3 sigma protein and human papillomavirus type 16 E6 in oral squamous cell carcinoma
|Bhawal UK,Sugiyama M,Nomura Y,Kuniyasu H,Tsukinoki K||Archives of otolaryngology--head and neck surgery (134:1055)||2008|
The p53 pathway and outcome among patients with T1G3 bladder tumors.
MA5-11663 was used in immunohistochemistry to study the prognostic value of p53 pathway expression in patients with T1G3 bladder tumors
|López-Knowles E,Hernández S,Kogevinas M,Lloreta J,Amorós A,Tardón A,Carrato A,Kishore S,Serra C,Malats N,Real FX||Clinical cancer research : an official journal of the American Association for Cancer Research (12:6029)||2006|
Expression and prognostic significance of 14-3-3sigma and ERM family protein expression in periampullary neoplasms.
MA5-11663 was used in immunohistochemistry to study the expression and prognostic significance of 14-3-3sigma and ERM family proteins in periampullary neoplasms
|Hustinx SR,Fukushima N,Zahurak ML,Riall TS,Maitra A,Brosens L,Cameron JL,Yeo CJ,Offerhaus GJ,Hruban RH,Goggins M||Cancer biology and therapy (4:596)||2005|
Novel snail1 target proteins in human colon cancer identified by proteomic analysis.
MA5-11663 was used in western blot to perform a proteomic study of Snail1-regulated events during epithelial-to-mesenchymal transition
|Larriba MJ,Casado-Vela J,Pendás-Franco N,Peña R,García de Herreros A,Berciano MT,Lafarga M,Casal JI,Muñoz A||PloS one (5:null)||2010|