Immunofluorescent analysis of 14-3-3 sigma showing staining in the cytoplasm of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a 14-3-3 sigma polyclonal antibody (Product # PA5-34644) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 248 of 14-3-3 sigma (Uniprot ID#P31947)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34644 targets 14-3-3 sigma in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and Mouse samples.
The PA5-34644 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 248 of 14-3-3 sigma (Uniprot ID#P31947).
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway .
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