Invitrogen

14-3-3 sigma Monoclonal Antibody (1433S01)

14-3-3 sigma Antibody in Immunocytochemistry (ICC/IF)

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14-3-3 sigma Antibody (MA5-11663) in ICC/IF

Immunofluorescence analysis of 14-3-3 sigma was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled 14-3-3 sigma (1433S01) Mouse Monoclonal Antibody (Product # MA5-11663) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at ... View More

14-3-3 sigma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

14-3-3 sigma Antibody in Western Blot (WB)

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Product Details

MA5-11663

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

Immunocytochemistry (ICC/IF)

1:10-1:200

Immunoprecipitation (IP)

2 µg/mL

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1433S01

Immunogen

Recombinant human 1433 sigma protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10987350

Product Specific Information

MA5-11663 targets 14-3-3 sigma in ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human samples.

The MA5-11663 immunogen is recombinant human 1433 sigma protein.

Target Information

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Activation of the Ca²⁺ sensing receptor and the PKC/WNK4 downstream signaling cascade induces incorporation of ZO-2 to tight junctions and its separation from 14-3-3.

Molecular biology of the cell

Amaya E,Alarcón L,Martín-Tapia D,Cuellar-Pérez F,Cano-Cortina M,Ortega-Olvera JM,Cisneros B,Rodriguez AJ,Gamba G,González-Mariscal L

Published figure using 14-3-3 sigma monoclonal antibody (Product # MA5-11663) in Western Blot

Thu Aug 15 00:00:00 EDT 2019

Hypoxia induces an undifferentiated phenotype of oral keratinocytes in vitro.

Cells, tissues, organs

Kato H,Izumi K,Uenoyama A,Shiomi A,Kuo S,Feinberg SE

Published figure using 14-3-3 sigma monoclonal antibody (Product # MA5-11663) in Western Blot

Mon Oct 05 00:00:00 EDT 2015

Identification of novel target proteins in sebaceous gland carcinoma.

Head & neck

Erovic BM,Al Habeeb A,Harris L,Goldstein DP,Kim D,Ghazarian D,Irish JC

MA5-11663 was used in immunohistochemistry to identify novel therapeutic target proteins in sebaceous gland carcinoma

Wed May 01 00:00:00 EDT 2013

14-3-3 sigma is a useful immunohistochemical marker for diagnosing ovarian granulosa cell tumors and steroid cell tumors.

International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists

Chen L,Yang B

MA5-11663 was used in immunohistochemistry to study the value of immunohistochemical expression of 14-3-3-sigma as a diagnostic marker of ovarian granulosa cell tumors and steroid cell tumors

Fri Mar 01 00:00:00 EST 2013

Prognostic and predictive markers in medullary thyroid carcinoma.

Endocrine pathology

Erovic BM,Kim D,Cassol C,Goldstein DP,Irish JC,Asa SL,Mete O

MA5-11663 was used in immunohistochemistry to identify markers with progniostic and predictive value in patients with medullary thyroid cancer

Sat Dec 01 00:00:00 EST 2012

Biomarkers of parathyroid carcinoma.

Endocrine pathology

Erovic BM,Harris L,Jamali M,Goldstein DP,Irish JC,Asa SL,Mete O

MA5-11663 was used in immunohistochemistry to establish a biomarker panel suitable for the identification of parathyroid carcinomas

Sat Dec 01 00:00:00 EST 2012

Novel snail1 target proteins in human colon cancer identified by proteomic analysis.

PloS one

Larriba MJ,Casado-Vela J,Pendás-Franco N,Peña R,García de Herreros A,Berciano MT,Lafarga M,Casal JI,Muñoz A

MA5-11663 was used in western blot to perform a proteomic study of Snail1-regulated events during epithelial-to-mesenchymal transition

Tue Apr 20 00:00:00 EDT 2010

Proteomic profiling of differential display analysis for human oral squamous cell carcinoma: 14-3-3 σ Protein is upregulated in human oral squamous cell carcinoma and dependent on the differentiation level.

Proteomics. Clinical applications

Hayashi E,Kuramitsu Y,Fujimoto M,Zhang X,Tanaka T,Uchida K,Fukuda T,Furumoto H,Ueyama Y,Nakamura K

MA5-11663 was used in immunohistochemistry to detect the expression of 14-3-3 sigma in oral squamous cell carcinoma

Sun Nov 01 00:00:00 EDT 2009

Immunostaining as an adjunct to cytology for diagnosis of pancreatic adenocarcinoma.

Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association

Agarwal B,Ludwig OJ,Collins BT,Cortese C

MA5-11663 was used in immunohistochemistry to study whether immunostaining as an adjunct to cytology improves the diagnosis of pancreatic adenocarcinoma

Mon Dec 01 00:00:00 EST 2008

Loss of 14-3-3 sigma protein expression and presence of human papillomavirus type 16 E6 in oral squamous cell carcinoma.

Archives of otolaryngology--head & neck surgery

Bhawal UK,Sugiyama M,Nomura Y,Kuniyasu H,Tsukinoki K

MA5-11663 was used in immunohistochemistry to study the role of 14-3-3 sigma protein and human papillomavirus type 16 E6 in oral squamous cell carcinoma

Wed Oct 01 00:00:00 EDT 2008

The clinicopathological and prognostic impact of 14-3-3 sigma expression on vulvar squamous cell carcinomas.

BMC cancer

Wang Z,Tropè CG,Suo Z,Trøen G,Yang G,Nesland JM,Holm R

MA5-11663 was used in immunohistochemistry to study the pathological and prognostic significance of 14-3-3 sigma expression fin vulvar squamous cell carcinomas

Fri Oct 24 00:00:00 EDT 2008

Promoter methylation of PARG1, a novel candidate tumor suppressor gene in mantle-cell lymphomas.

Haematologica

Ripperger T,von Neuhoff N,Kamphues K,Emura M,Lehmann U,Tauscher M,Schraders M,Groenen P,Skawran B,Rudolph C,Callet-Bauchu E,van Krieken JH,Schlegelberger B,Steinemann D

MA5-11663 was used in western blot to characterize a novel tumor suppressor gene and its role in mantle-cell lymphomas

Sun Apr 01 00:00:00 EDT 2007

Growth inhibitory effect of the human NIT2 gene and its allelic imbalance in cancers.

The FEBS journal

Lin CH,Chung MY,Chen WB,Chien CH

MA5-11663 was used in western blot to study the role of NIT2 gene in cell proliferation and tumorigenesis

Fri Jun 01 00:00:00 EDT 2007

The p53 pathway and outcome among patients with T1G3 bladder tumors.

Clinical cancer research : an official journal of the American Association for Cancer Research

López-Knowles E,Hernández S,Kogevinas M,Lloreta J,Amorós A,Tardón A,Carrato A,Kishore S,Serra C,Malats N,Real FX

MA5-11663 was used in immunohistochemistry to study the prognostic value of p53 pathway expression in patients with T1G3 bladder tumors

Sun Oct 15 00:00:00 EDT 2006

Identification of 14-3-3sigma as a contributor to drug resistance in human breast cancer cells using functional proteomic analysis.

Cancer research

Liu Y,Liu H,Han B,Zhang JT

MA5-11663 was used in western blot to study the role of 14-3-3sigma in drug resistance in human breast cancer cells

Wed Mar 15 00:00:00 EST 2006

Expression and prognostic significance of 14-3-3sigma and ERM family protein expression in periampullary neoplasms.

Cancer biology & therapy

Hustinx SR,Fukushima N,Zahurak ML,Riall TS,Maitra A,Brosens L,Cameron JL,Yeo CJ,Offerhaus GJ,Hruban RH,Goggins M

MA5-11663 was used in immunohistochemistry to study the expression and prognostic significance of 14-3-3sigma and ERM family proteins in periampullary neoplasms

Sun May 01 00:00:00 EDT 2005

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