|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from internal region of human 14-3-3 zeta|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:25|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, chicken, mouse and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil or breast carcinoma.
14-3-3 proteins are present as multigene families in most organisms. Signal-induced phosphorylation has the ability to change protein function. Sometimes, however, phosphorylation is not enough to change a proteinâ€™s function. 14-3-3 proteins play an important role of bringing signal transduction to completion. They regulate many cellular processes that are important in cancer biology, such as apoptosis and cell-cycle. checkpoints.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.