Immunofluorescence of Sea Urchin embryo cells stained for 4E-T/EIF4ENIF1. Cells were blocked with 0.01% BSA and stained with 4E-T/EIF4ENIF1 Polyclonal Antibody (Product # PA5-18235) at 1:25 dilution followed by a fluorophore-conjugated anti-goat IgG secondary antibody. Staining was visualized by confocal microscopy. 4E-T staining is depicted in green, staining for a proprietary antigen in red, and DAPI nuclear stain in blue. Data courtesy of the Innovators Program.
|Tested species reactivity||Echinoderm, Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (AKVISVDELEYRQ) corresponding to the C-terminus amino acids of EIF4ENIF1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.25-0.5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.