|Tested species reactivity||Chemical|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Modified base 5-methyl cytidine (5-MeCyd) is found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.