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Active Motif
| Applications | Tested Dilution | Publications |
|---|---|---|
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
Dot blot (DB) |
0.5-2 µg/mL | - |
ChIP-sequencing (ChIP-Seq) |
1-2 µg | - |
Methylated DNA Immunoprecipitation (MeDIP) |
1-2 µg | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Published species |
Mouse |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
A1 |
Immunogen |
This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to kLH and recognizes 5-Methylcytosine. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.5, with 30% glycerol |
Contains |
0.035% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2793653 |
Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 5-mC; 5-meC; 5-Methyl-cytosine; 5mc; C5H7NO3
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