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|Tested species reactivity||Chemical|
|Host / Isotype||Sheep / IgG|
|Immunogen||5-methyl cytosine molecule conjugated to KLH.|
|Storage buffer||PBS, pH 7.5|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-dependent|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||10 µg/ml|
|Immunoprecipitation (IP)||25-100 µg/ml|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-30675 detects 5-Methyl Cytosine from various samples.
PA1-30675 has been successfully used in immunohistochemistry (frozen tissue), immunoprecipitation, and Western blot applications. The use of 7mM NaOH in the IP is crutial.
The PA1-30675 immunogen is the 5-methyl cytosine molecule conjugated to KLH.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.