|Tested species reactivity||Tag|
|Host / Isotype||Mouse / IgG1|
|Immunogen||PAX6 transcription factor linked to histidine tag.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/10|
|Immunofluorescence (IF)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-81891 recognizes proteins containing the motiff H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts aginst all His-tagged proteins tested so far.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.