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|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||6x His synthetic peptide|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunofluorescence (IF)||1:500 - 1:2000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000 - 1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
MA1-21315 detects 6X-His Epitope Tag in samples.
MA1-21315 has been successfully used in ELISA, immunofluorescence, immunoprecipitation and Western Blot procedures.
MA1-21315 immunogen corresponds to 6x His synthetic peptide.
MA1-21315 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Autophagy enhances NFκB activity in specific tissue macrophages by sequestering A20 to boost antifungal immunity.
MA1-21315-1MG was used in immunoprecipitation to report that autophaghy regulates NFkapaB activity through A20 sequestration.
|Kanayama M,Inoue M,Danzaki K,Hammer G,He YW,Shinohara ML||Nature communications (6:null)||2015|
His Tag; Histidine Epitope Tag; Histidine Tag