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|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||6x His synthetic peptide: C-term -His-His-His-His-His-His-COOH|
|Contains||0.01% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
R933-25 detects 6x His tag (C-term) -His-His-His-His-His-His-COOH.
This antibody has been tested in IF experiments using cultured CHO (Chinese Hamster ovary) cells expressing recombinant epitope-tagged fusion proteins. For IF, dilute the FITC-conjugated antibody in PBS containing 10% fetal bovine serum (FBS). The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction.
This antibody has also been tested against purified Positope™ control protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
The minimal gene set member msrA, encoding peptide methionine sulfoxide reductase, is a virulence determinant of the plant pathogen Erwinia chrysanthemi.
R933-25 was used in western blot to study the role of MsrA in Erwinia chrysanthemi pathogenesis
|Hassouni ME,Chambost JP,Expert D,Van Gijsegem F,Barras F||Proceedings of the National Academy of Sciences of the United States of America (96:887)||1999|
His Tag, Histidine Tag, Histidine Epitope Tag