Invitrogen

ABCB5 Monoclonal Antibody (5H3C6)

Product Image Gallery

ABCB5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of HepG2 cells using ABCB5 monoclonal antibody (Product # MA5-17026) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

ABCB5 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ABCB5 monoclonal antibody (Product # MA5-17026) followed with DAB staining.

ABCB5 Antibody in Western Blot (WB) — Western blot analysis of ABCB5 using a ABCB5 monoclonal antibody (Product # MA5-17026) against a human ABCB5 recombinant protein.

ABCB5 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of HepG2 cells using ABCB5 monoclonal antibody (Product # MA5-17026) (green) and negative control (purple).

Product Details

MA5-17026

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:1,000	-
Immunocytochemistry (ICC/IF)	1:200-1:1,000	-
Flow Cytometry (Flow)	1:200 - 1:400	View 1 publication 1 publication
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	5H3C6
Immunogen	Purified recombinant fragment of human ABCB5 (amino acids: 481-674) expressed in E. Coli. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2538498

Product Specific Information

MA5-17026 targets ABCB5 in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17026 immunogen is purified recombinant fragment of human ABCB5 (amino acids: 481-674) expressed in E. Coli.

MA5-17026 detects ABCB5 which has a predicted molecular weight of approximately 89.8kDa.

Target Information

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

B4GALNT1 induces angiogenesis, anchorage independence growth and motility, and promotes tumorigenesis in melanoma by induction of ganglioside GM2/GD2.

Scientific reports

Yoshida H,Koodie L,Jacobsen K,Hanzawa K,Miyamoto Y,Yamamoto M

MA5-17026 was used in Flow Cytometry to perform functional analyses of B4GALNT1-overexpressing cells.

Mon Jan 27 00:00:00 EST 2020

A novel spheroid-based co-culture model mimics loss of keratinocyte differentiation, melanoma cell invasion, and drug-induced selection of ABCB5-expressing cells.

BMC cancer

Klicks J,Maßlo C,Kluth A,Rudolf R,Hafner M

MA5-17026 was used in Immunohistochemistry-immunofluorescence to describe a novel, simple spheroid-based melanoma tri-culture model composed of fibroblasts, keratinocytes, and melanoma cells which mimicked features observed in early melanoma stages.

Mon Apr 29 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: ABCB5 P-gp; ATP-binding cassette protein; ATP-binding cassette sub-family B member 5; ATP-binding cassette, sub-family B (MDR/TAP), member 5; P-glycoprotein ABCB5

Gene Aliases: ABCB5; ABCB5alpha; ABCB5beta; EST422562

UniProt ID: (Human) Q2M3G0

Entrez Gene ID: (Human) 340273

Molecular Function: ATP-binding cassette (ABC) transporter