|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||fusion protein of bacterial MBP and rat Mrp2, containing the 202-amino acid COOH terminal end|
|Storage buffer||tissue culture supernatant with 0.7% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent.|
|Immunocytochemistry (ICC)||1:20 - 1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:20 - 1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For immunohistochemistry (paraffin) applications, it is recommended to pretreat antibody with 0.01 M citric acid, pH 6.0, in distilled water at 100°C 3 times for 5 minutes.
M2III-5 reacts with an internal epitope of MRP2, a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.