Invitrogen

ABCG1 Recombinant Rabbit Monoclonal Antibody (SU03-26)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (14) ABCG1 antibodies

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ABCG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ABCG1 of paraffin-embedded Human lung tissue using a ABCG1 Monoclonal antibody (Product #MA5-32110). Counter stained with hematoxylin.

ABCG1 Antibody in Western Blot (WB) — Western blot was performed using Anti-ABCG1 Recombinant Rabbit Monoclonal Antibody (SU03-26)(Product # MA5-32110) and a ~110kDa band corresponding to ABCG1 was observed across cell lines and tissues tested. Membrane enriched extracts (50 µg lysate) of MCF-7 (Lane 1), MOLT-4 (Lane 2), Raji (Lane 3), HeLa (Lane 4) Mouse Lung (Lane 5) and Mouse Liver (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). A non specific band around 37kDa was observed across samples tested.

ABCG1 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using ABCG1 Recombinant Rabbit Monoclonal Antibody (SU03-26)(Product #MA5-32110), shows increased expression of ABCG1 in THP-1 cells treated with 50ng/ml TPA (24h) , THP-1 cells treated with (TPA-50 ng/mL-24h)+ (IL33-10 ng/mL-24h) and THP-1 cells treated with (TPA-50 ng/mL-24h)+TNF-a 10 ng/mL-48h) as compared to untreated THP-1 cells. {TM}

Product Details

MA5-32110

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	SU03-26
Immunogen	Synthetic peptide within Human ABCG1 aa 161-210 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809403

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. The ABC transporter ABCG1 (ABC8) is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages and is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. In contrast, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ABC transporter 8; ATP-binding cassette sub-family G member 1; ATP-binding cassette transporter 8; ATP-binding cassette transporter member 1 of subfamily G; ATP-binding cassette, sub-family G (WHITE), member 1; homolog of Drosophila white; MGC34313; White protein homolog; white protein homolog (ATP-binding cassette transporter 8)

Gene Aliases: ABC8; ABCG1; WHITE1; WHT1

UniProt ID: (Human) P45844

Entrez Gene ID: (Human) 9619, (Rat) 85264

Molecular Function: ATP-binding cassette (ABC) transporter primary active transporter transporter