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Western blot analysis of ABIN-1 was performed using whole cell extracts (30 ug lysate) of A-431 (Lane 1), PC-3 (Lane 2),HEL 92.1.7 (Lane 3) and K-562 (Lane 4). The blots were probed with Anti-ABIN-1 Mouse monoclonal Antibody (Product # 37-6100, 2 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/ml, 1:2500 dilution). A 72 kDa band corresponding to ABIN-1 was observed across the cell lines tested. A lower band of 48 kDa was observed showing NEMO/IKK gamma which binds in complex. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Synthetic peptide derived from the N-terminal region of the human, mouse and rat ABIN-1 protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP-or TRAF2-mediated transactivation signal. Increases cell surface CD4(T4) antigen expression. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Mouse||Not Cited||ABIN1 protein cooperates with TAX1BP1 and A20 proteins to inhibit antiviral signaling.||Gao L,Coope H,Grant S,Ma A,Ley SC,Harhaj EW||The Journal of biological chemistry (286:36592)||2011|
A20-binding inhibitor of NF-kappa-B activation 1; ABIN-1; HIV-1 Nef-interacting protein; NAF1; Nef-associated factor 1 SNP; nip40-1; VAN; virion-associated nuclear shuttling protein
ABIN-1; KIAA0113; NAF1; nip40-1; TNIP1; VAN