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Invitrogen
ACACA/ACACB Recombinant Rabbit Monoclonal Antibody (17C5)
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FIGURE: 1 / 14
ACACA/ACACB Antibody (MA572507) in IHC (P)
Immunohistochemistry analysis of ACACA/ACACB on paraffin-embedded Human Endometrial Cancer tissue. Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15 min. Samples were incubated with ACACA/ACACB Recombinant Monoclonal (Product # MA572507) using a dilution at 1:200 overnight at 4°C. Followed by DAB staining.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA572507
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
17C5
Immunogen
KLH conjugated synthetic peptide derived from human Acetyl CoA carboxylase.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
ACACA and ACACB encode acetyl CoA carboxylase alpha and beta, the rate limiting enzymes that convert acetyl CoA to malonyl CoA for de novo fatty acid synthesis and regulation of fatty acid oxidation. ACACA is primarily cytosolic in lipogenic tissues such as liver and adipose, supporting fatty acid and triglyceride production, while ACACB is enriched in oxidative tissues such as heart and skeletal muscle, producing malonyl CoA near mitochondria to inhibit fatty acid entry via CPT1 and thereby modulate beta oxidation. Both enzymes are large biotin dependent carboxylases with biotin carboxylase, carboxyl transferase, and biotin carrier domains, and they are tightly controlled by phosphorylation through AMPK, by allosteric activation from citrate, by feedback from long chain acyl CoA species, and by transcriptional programs governed by SREBP1 and ChREBP. Genetic variation and dysregulation of ACACA and ACACB influence metabolic disorders including obesity, type 2 diabetes, nonalcoholic fatty liver disease, and cardiovascular disease. Pharmacologic ACC inhibitors decrease malonyl CoA levels, reduce lipogenesis, and increase fatty acid oxidation, and are being developed for metabolic and liver indications. Detection and study use enzyme activity assays, metabolite profiling, immunoblotting, and CRISPR or RNA interference approaches.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ACAC; Acaca; ACACB-1; ACC-a; ACC-alpha; ACC-B; ACC-beta; ACC1; ACC2; ACCA; acetyl-CoA carboxylase (EC 6.4.1.2); acetyl-CoA carboxylase 265; acetyl-coenzyme A carboxylase (EC 6.4.1.2); acetyl-Coenzyme A carboxylase 2; acetyl-Coenzyme A carboxylase alpha; acetyl-Coenzyme A carboxylase beta; unnamed protein product
Gene Aliases: A530025K05Rik; ACAC; Acac1; ACACAD; ACACalpha; ACACbeta; ACC; ACC-beta; ACC1; ACC2; ACCA; ACCalpha; ACCB; ACCbeta; Gm738; hACC1; HACC275
UniProt ID: (Human) Q13085, (Human) O00763, (Mouse) Q5SWU9, (Rat) P11497, (Mouse) E9Q4Z2
Entrez Gene ID: (Human) 31, (Human) 32, (Mouse) 107476, (Rat) 60581, (Rat) 116719, (Mouse) 100705
acetyl-CoA metabolic process
fatty acid biosynthetic process
acyl-CoA metabolic process
lipid biosynthetic process
fatty-acyl-CoA biosynthetic process
protein homotetramerization
malonyl-CoA biosynthetic process
pentose-phosphate shunt
purine nucleotide metabolic process
response to nutrient
response to xenobiotic stimulus
positive regulation of lipid storage
regulation of glucose metabolic process
fatty acid oxidation
response to nutrient levels
negative regulation of fatty acid beta-oxidation
negative regulation of fatty acid oxidation
lactic acid secretion
negative regulation of lipid catabolic process
fatty acid homeostasis
positive regulation of heart growth
tricarboxylic acid metabolic process
intracellular aspartate homeostasis
glutamate homeostasis
energy homeostasis
glucose import across plasma membrane
regulation of cardiac muscle hypertrophy in response to stress
response to caloric restriction
tissue homeostasis
lipid metabolic process
lipid homeostasis
cellular response to prostaglandin E stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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