Invitrogen

ACOT12 Monoclonal Antibody (OTI1B9)

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ACOT12 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ACOT12 monoclonal antibody (Product # MA5-25428).

ACOT12 Antibody in Western Blot (WB) — Western blot analysis of ACOT12 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with ACOT12 (Product # MA5-25428) monoclonal antibody.

ACOT12 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 5 ACOT12 is the downstream target of miR-155-5p and inhibits glioma development. A Prediction of downstream target genes of miR-155-5p via four online databases (miRDB, TargetScan, miRTarBase); B Sequence alignment showing the potential binding sites between ACOT12 mRNA and miR-155-5p; Luciferase reporter activity of WT or MUT ACOT12 in 293 T cells co-transfected with miR-155-5p mimic; C , D Relative mRNA and protein levels of ACOT12 in glioma cells transfected with miR-155-5p inhibitor of mimic; E , F Relative expression level of ACOT12 in glioma cells transfected with si-ACOT12 alone or co-transfected with si-ACOT12 and miR-155-5p inhibitor detected by RT-qPCR and western blotting; G The Pearson correlation between miR-155-5p level and ACOT12 level was measured in glioma tissues. The DeltaCt values (normalized to GAPDH) were subjected to Pearson correlation analysis ( R = -0.43, P < 0.005); H Relative expression level of ACOT12 in glioma sections of different grades detected by IHC; I , J Migration and invasion of glioma cells transfected with miR-155-5p inhibitor or co-transfected with ACOT12 siRNA and miR-155-5p inhibitor, as detected by wound healing assays and Matrigel-coated Transwell assays. Representative images are shown. Data represent as the mean +- SEM from three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 (Student's t- test).

Product Details

MA5-25428

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Flow Cytometry (Flow)

1:100

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI1B9

Immunogen

Full length human recombinant protein of ACOT12 produced in HEK293T cell

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.91 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2722804

Target Information

Hydrolyzes acetyl-CoA to acetate and CoA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12.

Cell death & disease

Bao Z,Zhang N,Niu W,Mu M,Zhang X,Hu S,Niu C

MA5-25428 was used in Western Blot, Immunohistochemistry to show that exosomal miR-155-5p is also a potential predictive biomarker for glioma, which may provoke the development of novel diagnostic and therapeutic strategies against glioma.

Fri Aug 19 00:00:00 EDT 2022

Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12.

Cell death & disease

Bao Z,Zhang N,Niu W,Mu M,Zhang X,Hu S,Niu C

Fri Aug 19 00:00:00 EDT 2022

Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12.

Cell death & disease

Bao Z,Zhang N,Niu W,Mu M,Zhang X,Hu S,Niu C

Fri Aug 19 00:00:00 EDT 2022

Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12.

Cell death & disease

Bao Z,Zhang N,Niu W,Mu M,Zhang X,Hu S,Niu C

Fri Aug 19 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: Acetyl-coenzyme A thioesterase; Acyl-CoA thioester hydrolase 12; Acyl-CoA thioesterase 12; Acyl-coenzyme A thioesterase 12; CACH-1; Cytoplasmic acetyl-CoA hydrolase 1; cytosolic acetyl-CoA hydrolase; hCACH-1; StAR-related lipid transfer (START) domain containing 15; StARD15; START domain-containing protein 15

Gene Aliases: ACOT12; CACH; CACH-1; CACH1; STARD15; THEAL

UniProt ID: (Human) Q8WYK0

Entrez Gene ID: (Human) 134526

Molecular Function: esterase

Function(s)

acetyl-CoA metabolic process pyruvate metabolic process fatty acid metabolic process acyl-CoA metabolic process long-chain fatty-acyl-CoA biosynthetic process

Process(es)

acetyl-CoA hydrolase activity ATP binding lipid binding acyl-CoA hydrolase activity carboxylic ester hydrolase activity

ACOT12 Monoclonal Antibody (OTI1B9)

Product Details

MA5-25428

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

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