|Tested species reactivity||Human|
|Host / Isotype||Insect|
|Storage buffer||TBS, pH 7.5, with 0.05% Brij-35, 5mM CaCl2, 50mM Imidazole|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
RP-77529 is a purified recombinant human ADAMTS5 D625-930, produced with the baculovirus expression system and purified from insect cell culture supernatants. The protein contains the catalytic domain, the disintegrin domain and the thrombospondin type-1 motif of full-lenth ADAMTS5 followed by a C-terminal His6-tag. The calculated MW of the amino acid sequence is 40.8 kDa. In SDS-PAGE the protein exhibits a MW of about 50 kDa. The higher Mr is probably due to glycosylation. RP-77529 may be used as a control with corresponding anti-ADAMTS5 antibodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.