Immunofluorescent analysis of ADAR in HeLa cells fixed in 4% paraformaldehyde for 10 min, using an aDAR monoclonal antibody (Product # MA5-17285). Green: Primary antibody at a dilution of 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 µm.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant fragment contains a sequence corresponding to a region within amino acids 472 and 757 of ADAR1|
|Storage buffer||PBS, pH 7|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17285 targets ADAR in WB applications and shows reactivity with Human samples.
The MA5-17285 immunogen is recombinant fragment contains a sequence corresponding to a region within amino acids 472 and 757 of ADAR1.
ADAR catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.