Invitrogen

ADH1A/ADH1B/ADH1C Polyclonal Antibody

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Datasheet

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ADH1A/ADH1B/ADH1C Antibody in Western Blot (WB) — Western blot analysis of extracts of Mouse lung using ADH1A/ADH1B/ADH1C Polyclonal Antibody (Product # PA5-116865) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

ADH1A/ADH1B/ADH1C Antibody in Immunohistochemistry (IHC) — Immunohistochemistry/Immunofluorescence analysis of ADH1A/ADH1B/ADH1C in Rat liver tissue using ADH1A/ADH1B/ADH1C Polyclonal Antibody (Product # PA5-116865) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (IHC)

1:50-1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunofluorescence (IF)

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 280 to the C-terminus of human ADH1A/ADH1B/ADH1C

Conjugate

Unconjugated

Form

Liquid

Concentration

2.47 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Positive Samples: Mouse lung

Target Information

Alcohol dehydrogenase,consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism.Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ADH, alpha subunit; ADH, beta subunit; ADH, gamma subunit; alcohol dehydrogenase 1 (class I), alpha polypeptide; Alcohol dehydrogenase 1A; Alcohol dehydrogenase 1B; Alcohol dehydrogenase 1C; alcohol dehydrogenase 2 (class I), beta polypeptide; alcohol dehydrogenase 3 (class I), gamma polypeptide; Alcohol dehydrogenase subunit alpha; Alcohol dehydrogenase subunit beta; Alcohol dehydrogenase subunit gamma; aldehyde reductase; All-trans-retinol dehydrogenase [NAD(+)] ADH1B; epididymis secretory protein Li 117

Gene Aliases: ADH1; ADH1A; ADH1B; ADH1C; ADH2; ADH3; HEL-S-117

UniProt ID: (Human) P07327, (Human) P00325, (Human) P00326

Entrez Gene ID: (Human) 124, (Human) 125, (Human) 126

Molecular Function: dehydrogenase oxidoreductase reductase